Lasers

Laser

Power (mW)

Wavelength (nm)

Example fluors

Near UV

35

405

DAPI, Hoechst, Cascade Blue/Yellow, most UV-excitable dyes

Argon

25

458

LuY, BOBO-1, CFP

488

Cy2, GFP, FITC, Alexa Fluor 488, PE, YOYO-1

514

YFP

HeNe1

1

543

PE, Alexa Fluor 546, rhodamine, Cy3, DsRed, PI

HeNe2

2

594

Alexa Fluor 594, Cy3.5, Texas Red

HeNe3

5

633

Cy5, Alexa Fluor 633, Alexa Fluor 647