Biotin: The role of a human vitamin in malaria parasites
Dr. Sean T. Prigge; Professor; Johns Hopkins Bloomberg School of Public Health
March 16 at 12:20pm in the Fralin Auditorium, Fralin Hall room 102
Hosted by Dr. M. Klemba
Malaria parasites require certain host nutrients for growth and survival. In this project, we examined the role of the human vitamin biotin in all stages of the malaria life cycle. We cultured blood and liver stage malaria parasites in the absence of biotin, and found that while blood stage replication was unaffected, liver stage parasites deprived of biotin were no longer capable of establishing a blood stage infection. Interestingly, biotin depletion resulted in more severe developmental defects than the genetic disruption of parasite biotin metabolism. This finding suggests that host biotin metabolism also contributes to parasite development. Since neither the parasite nor the human host can synthesize biotin, parasite infectivity may be affected by the nutritional status of the host.