Fralin Imaging Center
Zeiss LSM 880 Confocal Laser Scanning Microscope
The Zeiss LSM 880 with Airyscan is a confocal laser scanning microscope operated as a fee-for-use instrument in the Fralin Imaging Center, located in Fralin Hall.
Confocal microscopy offers the abilities to control the thickness of the sample that is imaged, eliminate out-of-focus light, and collect serial optical sections.
Samples can be live, fixed, or inanimate. The LSM 880 is equipped for live cell imaging, sub-micron resolution imaging with Airyscan/Airyscan FAST scanning, and capturing reflected as well as fluorescent images.
Features include: ·
- Inverted microscope stand with incubation chamber
- Detectors: 32-channel GaAsP array, 2 standard PMTs, Airyscan
- Laser lines: 405, 458, 488, 514, 543, 594, and 633 nm
- Objective lenses: 5x, 10X, 25X, 40X, 63X, 100x
- Definite Focus 2 to compensate for focal drift during long experiments
- Z-stacking and tile scanning
- Temperature and CO2 control modules for live cell imaging
- Motorized stage with piezo z stage
- Stage inserts for slides, multiwell plates, and small culture dishes
- Transmitted light detector
- Directly connected workstation with full Zeiss Zen Blue software for image processing
Zeiss Axio Observer Z1 Inverted Microscope
The Zeiss Axio Observer is an inverted microscope offering both fluorescent and brightfield imaging. The system features a manual stage with inserts for slides or multiwell dishes, objective lenses from 10x to 100x, filter sets for DAPI, FITC, Rhod, PI, and an AxioCam MRc camera. System software is Zeiss Zen Blue.
The microscope is available at no charge for independent use by researchers, with an instructional video available.
Microcallus derived from a regenerating Arabidopsis mesophyll protoplast (single leaf cell) expressing nuclear markers for auxin (mScarlet) and cytokinin (mNeonGreen). The fluorescent image is a projection of a Z-stack obtained with a Zeiss LSM 880. Images courtesy of Bas Bargmann
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