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Equipment and Rates

Nikon SoRa

The Nikon SoRa (super resolution by optical reassignment) is a spinning disk confocal built around the inverted Ti2E, with additional resolution provided by the SoRa microlens disk and magnifiers, improving lateral resolution to 120nm.

The system has 7 excitation lasers available (405 / 445 / 488 / 514 / 561 / 594 / 640) as well as a photostimulation unit, and has two back-thinned sCMOS cameras for dual capture capabilities. This versatile system can be used for gentle, high-speed transmitted light, widefield, confocal, and high-resolution imaging.

Nikon AXR with NSPARC and STEDYCON

The Nikon AXR is a point scanning confocal built around the inverted Ti2E, with additional resolution provided by the NSPARC array detector which improves signal-to-noise. With the largest field of view available on the market and the custom design, the AXR is capable of providing a wide range of imaging.

It also has a super resolution Abberior STEDYCON (stimulation emission depletion) module, with lateral resolution down to 30nm.

The confocal side of the system has 8 laser lines available (405 / 445 / 488 / 514 / 561 / 594 / 640 / 730) and the STEDYCON is capable of 4-color imaging, with 3-color STED potential with its 775 nm depletion laser.

Analysis Software

Nikon’s NIS Analysis with General Analysis 3, Batch Denoise, and Batch Deconvolution software packages are available on a shared, free-to-use workstation PC in Steger Hall. Though geared towards Nikon images, TIF files are also able to be processed and analyzed here. This workstation PC also has ZenLite and FIJI software for other image processing and analysis needs.

Rate Information

  • Hourly rates for internal use of the instruments are $40/hr and facility assistance rates, for imaging and training, are an additional $50/hr.
  • Please contact Emery Ng for external rate information or to request a consultation imaging session free of charge.
  • Consumables and dyes offered for purchase to users and are price-adjusted based on the cost of the individual unit.