Nikon AXR with NSPARC and STEDYCON
Overview
The Nikon AXR is a point scanning confocal built around the inverted Ti2E, with additional resolution provided by the NSPARC APD array detector which improves signal-to-noise and extends resolution, and a super resolution Abberior STEDYCON module, with lateral resolution down to 30nm.
Specifications
Microscope
- Nikon inverted Ti2E with PerfectFocus gen. 4
- Prior piezo z stage with 600µm range
- Okolab stagetop incubator
Laser units
- LUA-S4 and LunF-combiner 405 / 445 / 488 / 515 / 561 / 594 / 640 / 730
Objective Lenses
| Mag | NA | W.D. (mm) | Imm | Corr |
|---|---|---|---|---|
| 2X* | 0.1 | 8.5 | Air | Plan Apo Lambda D |
| 10X | 0.45 | 4 | Air | Plan Apo Lambda D |
| 20X* | 0.8 | 0.8 | Air | Plan Apo Lambda D |
| 20X | 0.95 | 0.99 - 0.9 | WI | Apo LWD Lambda S |
| 25X | 1.05 | 0.55 | Sil | Plan Apo Lambda S |
| 40X | 1.15 | 0.61 - 0.59 | WI | Apo LWD Lambda S |
| 60X | 1.42 | 0.15 | Oil | Plan Apo Lambda D |
| 100X | 1.45 | 0.13 | Oil | Plan Apo Lambda D |
*Available to be put in instrument, please contact Emery to request use. Use the 100X for optimal STED resolution.
Detector units
- DUX with 3 GaAsP and 1 IR-PMT detectors
- NSPARC array detector of 25 APDs with down to 100 nm lateral x 300 nm axial resolution
Software
- NIS Elements confocal package with General Analysis 3 and 2D/3D Deconvolution tools
Abberior STED module
The STEDYCON unit is a convenient, shoebox version of Abberior's STED instruments. It is capable of acquiring up to 3 STED channels with one STED laser, given a long Stoke's shift green (488 nm excitation) dye is used, improving lateral resolution down to 30 nm. STED can be performed on a variety of fixed and live samples using appropriate dyes. A table of verified STED dyes is shown below.
STEDYCON with STEDYFOCUS
- Alignment-free design
- Excitation lasers: 405 (CW); 488 / 561 / 640 nm pulsed
- STED laser: 775 nm pulsed
- Scanner: QUAD beam, 90µm x 80µm FOV (at 100X) with speed up to 1 FPS
- Detectors: time gated APDs
- Browser-based software that runs on module for uninterrupted imaging
A 2D view of a diffraction-limited excitation point (blue) overlapped by a STED donut (orange) is shown on the upper left, with a graphical representation of the gaussian overlap below. The effective fluorescent spot and graph are shown on the right in green.
