Nikon AXR with NSPARC and STEDYCON
Overview
The Nikon AXR is a point scanning confocal built around the inverted Ti2E microscope, with additional resolution provided by the NSPARC APD array detector, which improves signal-to-noise and extends resolution, and a super resolution Abberior STEDYCON module, with lateral resolution down to 30nm.
Specifications
Microscope
- Nikon inverted Ti2E with PerfectFocus gen. 4
- Prior piezo z stage with 600µm range
- Okolab stagetop incubator
Laser units
- LUA-S4 and LunF-combiner 405 / 445 / 488 / 515 / 561 / 594 / 640 / 730
Objective Lenses
| Mag | NA | W.D. (mm) | Immersion | Corr |
|---|---|---|---|---|
2X* |
0.1 |
8.5 |
Air |
Plan Apo Lambda D |
10X |
0.45 |
4 |
Air |
Plan Apo Lambda D |
20X* |
0.8 |
0.8 |
Air |
Plan Apo Lambda D |
20X |
0.95 |
0.99 - 0.9 |
WI |
Apo LWD Lambda S |
25X |
1.05 |
0.55 |
Sil |
Plan Apo Lambda S |
40X |
1.15 |
0.61 - 0.59 |
WI |
Apo LWD Lambda S |
60X |
1.42 |
0.15 |
Oil |
Plan Apo Lambda D |
100X |
1.45 |
0.13 |
Oil |
Plan Apo Lambda D |
*Available to be put in instrument, please contact Emery to request use. Use the 100X for optimal STED.
Detector units
- DUX with 3 GaAsP (2 tunable) and 1 IR-PMT detectors
- NSPARC array detector of 25 APDs with down to 100 nm lateral x 300 nm axial resolution
Software
- NIS Elements confocal package with General Analysis 3 and 2D/3D Deconvolution tools
Nikon Confocal Comparison
Check the Resources tab for information comparing the confocal microscopes!
Abberior STED module
The STEDYCON unit is a convenient, shoebox version of Abberior's STED instruments. It is capable of acquiring up to 3 STED channels with one STED laser, given a long Stoke's shift green (488 nm excitation) dye is used, improving lateral resolution down to 30 nm. STED can be performed on a variety of fixed and live samples using appropriate dyes. A table of verified STED dyes is shown below.
STEDYCON with STEDYFOCUS
- Alignment-free design
- Excitation lasers: 405 (CW); 488 / 561 / 640 nm pulsed
- STED laser: 775 nm pulsed
- Scanner: QUAD beam, 90µm x 80µm FOV (at 100X) with speed up to 1 FPS
- Detectors: time-gated APDs
- Browser-based software that runs on module for uninterrupted imaging
A 2D view of a diffraction-limited excitation point (blue) overlapped by a STED donut (orange) is shown on the upper left, with a graphical representation of the gaussian overlap below. The effective fluorescent spot and graph are shown on the right in green.
