Zeiss LSM 880

An inverted Zeiss microscope with incubation chamber and a LSM 880 scanhead on the left port are shown to the left, and to the right an example monitor with ZEN software is shown.

Overview

The Zeiss LSM 880 with Airyscan is a laser scanning confocal microscope built around an inverted Zeiss Z1 located in Fralin Hall. This microscope can image live, fixed, and inanimate samples and is equipped with capabilities for live cell imaging, sub-micron resolution imaging with Airyscan/Airyscan FAST scanning, and capturing reflected as well as fluorescent images.

Specifications

Microscope

Inverted Zeiss Observer with Definite Focus 2
Piezo z stage
Incubation chamber

Laser Units

Near UV, Argon, and HeNe units for: 405 / 458 / 488 / 514 / 543 / 594 / 633

Objective Lenses

Mag	NA	W.D. (mm)	Imm	Corr
5X	0.25	12.5	Air	Flaur
10X	0.45	2	Air	Plan Apo
25X	0.8	0.57	IMM (WI, Gly, Oil)	Plan Apo
40X	1.2	0.28	WI	Apo
63X	1.15	0.6	WI	Apo
63X	1.4	0.19	Oil	Plan Apo
100X	1.46	0.11	Oil	Alpha Plan Apo

Detector units

2 standard MA PMTs
32 GaAsP PMT array for spectral detection and Airyscan

Software

Zeiss ZEN Blue for image acquisition, plus a separate workstation with Zeiss ZEN Blue and Black for image processing and analysis