Nikon SoRa

Overview

The Nikon SoRa (super resolution by optical reassignment) is a spinning disk confocal built around the inverted Ti2E, with enhanced resolution provided by the SoRa microlens disk and magnifiers, improving lateral resolution to 120nm. This versatile system is also equipped with a new photostimulation unit, and can be used for gentle, high-speed transmitted light, widefield, confocal, and super resolution imaging.

Specifications

Microscope

• Nikon inverted Ti2E with PerfectFocus gen. 4
• Prior piezo z stage with 450µm range
• Okolab Lauda cooling and heating stagetop incubator

Laser unit

• LunF-XL 405 / 445 / 488 / 514 / 561 / 594 / 640

Filters

• Standard quad (DAPI / GFP / RFP / Cy5)
• mCherry tri (CFP / YFP / mCherry)
• Standard tri (DAPI / GFP / RFP)

Objective Lenses

• 20x/0.8 Plan Apochromat Lambda D, DIC
• 40x/1.25 Plan Apochromat Lambda D silicone, DIC
• 60x/1.42 Plan Apochromat Lambda D oil, DIC
• 60x/1.49 Apochromat TIRF, oil

Cameras

• 2 Hamamatsu Orca-FusionBT C15440 sCMOS
• 16-bit
• 6.5µm x 6.5µm pixel size
• 21.1mm FOV

Software

• NIS Elements Advanced Research (AR), NIS.ai module, deconvolution suite