Nikon Spinning Disk SoRa
The Nikon SoRa (super resolution by optical reassignment) is a spinning disk confocal built around the inverted Ti2E, with enhanced resolution provided by the SoRa microlens disk and magnifiers, improving lateral resolution down to 120nm. This versatile system is also equipped with a photostimulation unit, and can be used for gentle, high-speed transmitted light, widefield, confocal, and extended resolution imaging.
Microscope
- Nikon inverted Ti2E with PerfectFocus gen. 4
- Prior piezo z stage with 450µm range
- Okolab Lauda cooling and heating stagetop incubator
Laser unit
- LunF-XL 405 / 445 / 488 / 514 / 561 / 594 / 640
Photostimulation laser unit
- LunF 405/473
Dichroic mirrors
- Standard quad (405/488/561/640)
- mCherry tri (445/514/594)
- Standard tri (405/488/594)
Detectors
- 2 Hamamatsu OrcaFusion BT sCMOS cameras with 6.5-micron pixel size and 3 scan modes
| Mag | NA | W.D. (mm) | Immersion | DIC | Corr |
|---|---|---|---|---|---|
| 20X | 0.8 | 0.8 | Air | No | Plan Apo Lambda D |
| 40X | 1.25 | 0.3 | Sil | Yes | Plan Apo Lambda D |
| 60X | 1.42 | 0.15 | Oil | No | Plan Apo Lambda D |
| 60X | 1.49 | 0.16 - 0.07 |
Oil | Yes | Apo TIRF |
NIS Elements Advanced Research (AR) is used for acquisition. The offline Nikon workstation in Steger Hall also includes General Analysis 3, batch denoise, and batch 2D/3D deconvolution tools.