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FAIM Microscope-Specific Resources

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Comparing Instruments

The Facility offers a free, self-paced online FIJI/ImageJ for Beginners course on Canvas.

In addition to an introduction to FIJI and basic commands, learners will also:

  • Make basic measurements
  • Use segmentation to make measurements
  • Perform background subtraction and other image math
  • Visualize 3D images
  • Perform basic 3D measurements

Outside of the core modules, specific PlugIn modules also offered include:

  • Trainable segmentation and subsequent measurements with Trainable Weka Segmentation and Labkit
  • Tracking over time with Trackmate
  • An introduction to ImageJ Macro creation for batch processing and analysis
  • Colocalization tools (pixel- and object-based) with Coloc2 and JaCoP

To be added to the course or ask questions, email flsimicroscopy@vt.edu with "FIJI Course" as the subject.

ImageJ for Beginners Syllabus

Imaging Glass Guide
Image Histogram and Display Guide
Signal Overlap and Associated Checks Guide
Changing Signal: Laser Power and Detector Gain Guide

Technical Recordings

Nikon's laser scanning vs. spinning disk confocal microscopy comparison can be found here.

Zeiss's Microscopy & Sample Prep 101, 2026 workshop sessions one and two will be uploaded shortly!

2025 Zeiss Lattice Lightsheet Talk & Workshop
Nikon TIRF Demo June 2025
2025 In-Person ImageJ for Beginners Workshop
2024 STED Workshop
Lattice Lightsheet Demo August 2024