General Microscopy Resources
Free online resources about microscopy fundamentals and image processing/analysis:
Learning Microscopy Fundamentals:
ThermoFisher's SpectraViewer for comparing fluorescence spectra, seeing what combinations will work, and what lasers will be usable!
Nikon's MicroscopyU: Reading materials and interactive tutoritals
Zeiss's Campus: Reading materials and interactive tutorials
iBiology Microscopy Course Videos: Includes image formation, detection, and specialized techniques
Oxford Instruments Microscopy Training Course: Short videos ranging from background to advanced techniques.
Baylor also has a short introduction to confocal video.
Global BioImaging (GBI) has a free, online mini course on Light Microscopy Technologies.
GBI's MicroTutor offers introductory information and practical knowledge on light microscopy experiments.
OpenCourseWare (MIT) course on Optics for anyone interested in the physics behind ray tracing and image formation.
"I have questions that need answers" - visit the image.sc forum!
FIJI (is just ImageJ) - a good workhorse that has a lot of basic tools and plugins. Email flsimicroscopy@vt.edu to be added to the free, self-paced, online ImageJ for Beginners course offered by the Facility!
ZenLite - Zeiss's free version of Zen with limited capabilities. Register to access.
CellProfiler - useful for segmentation pipelines.
QuPath "quantitative pathology" - generally used for tissue slices/whole slide images.
napari - python-based image processing and analysis.